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level: disha questions

Questions and Answers List

level questions: disha questions

Question	Answer
human genome project is closely associated with the rapid development of a new area in biology called as	bioinformatics
genes of interest can be selected from a genomic library by using	DNA probes
what are palindromic base sequence	palindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction
plasmids are the extrachromosomal part in the bacteria and are useful in	recombinant DNA technology
plasmids are extra chromosomal material possesing single stranded DNA, they are present in	in eukaryotes as well as in prokaryotes
in recombinant DNA technology, recombinants DNA are usualy transferred to E.coli, yeat beacuse of their rapid multiplication	this technology is employed for combining DNA from two different organisms to produce recombinant DNA
restriction enzymes cut the strand of DNA to produce sticky ends.(named so because they form h bond with theiir complemetary counter parts)	the stickness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.
the DNA fragments separated on an agarose gel can be visualised after staining with	ethidium bromide
a gene whose expression helps to identify transformed cell is known as	selectable marker
some features of plasmids	independent replication, circular structure, transferable
the taq polymerase enzyme is obtained from	thermus aquaticus
the dna molecule to which the gene of interest is integrated for cloning is called	vector
an analysis of chromosomal DNA using the southern hybridisation technique uses	electrophoresis, blotting and autoradiology
during the proccess of isolation of DNA chilled ethanol is added to	precipitate DNA
the colonies of recombinant bacteria appears white in contrast to blue colonies of non-recombinant bacteria because of	non-recombinant bacteria containing beta-galactosidase
genes of interest can be selected from a genomic library by using	DNA probes
stirred tank bioreactors hav been designed for	availability of oxygen throughout the process
the linking of antibiotic resistance gene with the plamid vector became possible with	DNA ligase
chromosomes in a bacterial cell can be 1-3 in number and	are always circular
the plasmids present in bacterial cells are	circular double helical DNA molecules
in vitro clonal propagation in plants is characterised by	PCR and RAPD
PCR and restriction fragment length polymorphism are the methods for	genetic fingerprinting
at what temp. does the DNA polymerase used in PCR remain active	at high temperature
for transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of	gold or tungsten
biolistics(gene gun) is suitable for	constructing recombinant DNA by joining with vectors