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level: TERMINOLOGIES

Questions and Answers List

level questions: TERMINOLOGIES

Question	Answer
Method of analysis which deal with MEASUREMENT OF SPECTRA of EMS In Pharmaceutical analysis: 1. measurements of the position (wavelength) in the electromagnetic spectrum where radiant energy has interacted with a chemical species 2. measurement of the power of transmitted, fluorescent, reflected or emitted energy	SPECTROMETRY
BRANCH OF SPECTROMETRY which embraces the MEASUREMENT OF THE ABSORPTION, by chemical species of RE of definite and narrow wavelength approximating monochromatic radiation	SPECTROPHOTOMETRY
Radiation of single wavelength 0.5 to 10 nm	MONOCHROMATIC RADIATION
The LENGTH of a complete WAVE or cycle, from the peak of one wave to the peak of the next	WAVELENGTH (λ)
Units of λ measurement: 1 µm = 2 nm = 3 Å =	. 1) 10^ -4 cm 2) 10^ -7 cm 3) 10^ -8 cm
Number of WAVES PER CM Equal to 1 / λ cm	WAVENUMBER (v)
Number of COMPLETE CYCLES which pass a given point per second (cps) Also expressed in Hertz where 1 Hertz = 1 cps	FREQUENCY
Functional group which absorbs radiant energy in the UV or VIS regions (ketones, aldehydes etc.)	CHROMOPHORE
Branch of spectrophotometry in which the absorption measurement is made in VIS region of the SPECTRUM. - fewer compounds absorbed in the visible region - specific for functional groups - conjugated- ↑ molar absorptivity and sensitivity of method	COLORIMETRY
Use to designate INSTRUMENTS which have RE - dispersing device such as PRISM OR GRATING, which permits the measurement of wavelength and radiant power. The color and intensity of transmitted RE are a measure of concentration and are determined by color comparison or more accurately using THIS	SPECTROPHOTOMETER/ SPECTROMETER
INSTRUMENT which have the necessary electronics to permit measurement of radiant power but which use a FILTER instead of a prism or diffraction grating for the purpose of increasing the sensitivity of measurement. a quantitative technique which makes use of the INTENSITY IN COLOUR of a solution is directly related to the concentration of the coloured species in it	COLORIMETER/ FILTER PHOTOMETER
Analytical METHOD based on ABSORPTION of radiant energy (RE) this method that utilize the visible region of the spectrum are called colorimetric methods Ex. color chart, analysis of pesticides/ glucose	ABSORPTIMETRIC METHOD