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Questions and Answers List

Question	Answer
1 chroma means __ 2 graphein means __	1 color 2 to write
A technique exploiting the interaction of the components of a mixture with a STATIONARY PHASE and a MOBILE PHASE (solvent) in order to SEPARATE the components. Components are separated by different levels of ADSORPTION to the stationary phase and SOLUBILITY in the mobile phase.	CHROMATOGRAPHY
HE WAS THE FIRST TO Separate various plant pigments thru glass column packed w/ calcium carbonate Separated as colored bands	Mikhail Tswett (1906) botanist
CHROMATOGRAPHY PHASES	1 Stationary phase – fixed phase 2Mobile phase
POROUS OR FINELY DIVIDED SOLID OR LIQUID that has been coated in thin layer on an inert supporting material Particles as SMALL and HOMOGENEOUS to provide a large surface area so that sorption and desorption of solutes will occur efficiently	Stationary phase
May be a pure liquid or mixture of solutions (buffers) or a gas (pure or homogeneous mixture)	Mobile phase
1 the material placed in the column to ADSORB the drug 2 the solvent to WASH the column 3 the liquid AFTER WASHING or the effluent liquid	1 Adsorbents 2 Eluants 3 Eluate
Classification of Chromatographic Methods:	1 Adsorption 2 Partition 3 Ion exchange 4 Size exclusion 5 Affinity chromatography PAASI ko