level: Vmax and Km
Questions and Answers List
level questions: Vmax and Km
| Question | Answer |
|---|---|
| what is Km | which is used to compare the affinity of different enzymes for their substrates. |
| Enzyme is saturated with substrate (Vmax) | Reaction rate is measured at different substrate concentrations, |
| Keeping enzymes concentration constant. (Vmax) | As substrate concentration is increased, reaction rate rises until the reaction reaches its maximum rate. |
| what does Km stand for | Michaelis-Menten constant. Substrate concentration where enzyme works at half its maximum rate |
| at 1/2 Vmax.... | Half of the active sites of enzyme are occupied by substrate. |
| the higher the affinitiy (likeliness that the substrate will bind to the active site).... | the lower this substrate concentration will be. Lower Michaelis-Menten constant. Faster maximum rate of reaction More likely that the product will be formed when a substrate molecule enters the active site. Less substrate needed |
| so the lower affinity = | a higher km |