O Steam autoclave sterilization

O steam a moist heat rapidly kills microorganism. –As steam fills the chamber the cooler air is pushed from an escape valve. –It isthe heat not the pressure that kills–disadvantage: rust -that kills –disadvantage: rust –Usually operates on four cycles: heat up,sterilizing, depressurization, drying§ Packaging materials may be fabric, but arecommonly film and paper pouches, nylon tubing, –temp: 250–273 –time 3–30