Explain how to use an optical microscope to view a prepared slide in four practical steps and two data collecting steps

1. Firstly place the slide onto the stage and use the clips to hold it in place 2. Then select the lowest-power objective lens (usually 4x) and turn the coarse focussing lens so the objective lens is almost touching the slide 3. Now look down the eyepiece and adjust the coarse focussing lens until the cell comes into focus, then turn the fine focussing lens so the cell comes into a clear-focus 4. Calculate the total magnification using the equation: Magnification (of the cell) = magnification of eyepiece lens x magnification of objective lens (Usually 10x x 4x ) = 40x 5. Lastly select a high power objective lens and adjust the fine focussing lens to bring the cells back into focus and record new results 6. After the practical you will be required to draw the cell sample with a magnification scale How to draw a magnification scale - place a ruler over the stage and measure the diameter of the field view in millimetres and present this measurement as a scale bar on your drawing and write down the magnification (eg. 100x)