What are the first four steps of the effects of pH on enzymes practical?

The first four steps of the practical: effect of pH on enzymes are First, place one drop of iodine solution into each well of a spotting tile Next take 3 test tubes. In the first test tube out 2cm cubed of starch solution, in the second hit 2cm cubed of amylase solution and in the third put 2cm cubed of pH 5 buffer solution (buffer solution is used to control the pH in biology) In the third step you place the test tubes in a water bath of 30 degrees Celsius and leave them for 10 minutes to allow the solution to reach the correct temperature Now combine the three solutions into one test tube and mud with a stirring rod then immediately return the test tube to the water bath and start a stopwatch