FS HN 420: ONPG (o-nitrophenyl β-D-galactopyranoside)

Question

ONPG (o-nitrophenyl β-D-galactopyranoside)

Kate Jaros · Accepted Answer

- ONPG is degraded by E. coli β-galactosidase to o-nitrophenyl β-D-galactopyranoside
- Nitrophenyl moiety is released and gives a yellow color measured at 420 nm

FS HN 420

ONPG (o-nitrophenyl β-D-galactopyranoside)

- ONPG is degraded by E. coli β-galactosidase to o-nitrophenyl β-D-galactopyranoside - Nitrophenyl moiety is released and gives a yellow color measured at 420 nm

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