Targeting the proteasome and destruction

1. Polyubiquitylated proteins bind the 19S regulatory particle of the proteasome 2. The RP uses ATP to generate energy to unfold the target protein and feed it into the 20S core. Deubiquitylases (DUBs) remove Ub molecules and return them to a common pool for recyclin. 3. Three proteolytic activities are encoded by the β subunits of the 20S core 4. The target protein is degraded into small peptides, which are ejected from the proteasome.