What are recommended methods to sacrifice rodents?

- overdose of injectable agents (sodium pentobarbital) - overdose of inhalational agents (halothane) - inhalation of carbon dioxide gas (con: creates stress, increased HPA axis) - inhalation of carbon monoxide gas (con: dangerous to humans) - decapitation (con: brain activity still present after 14s) - cervical dislocation - blunt force trauma (con: skilled lab personnel needed) - focus beam microwave irradiation, heating of the rodent's brain