What are applications of flow cytometry?

- immunophenotyping: simultaneously analysing mixed populations of immune cells for multiple parameters, makes use of fluorophore-conjugated antibodies that target specific surface markers on immune cells. - proliferation analysis: cells are stained with proliferation dye. when cells proliferate, the dye will be subdued. when cells do not proliferate, cells will remain bright with dye. - apoptosis: stain cells with dye that can only enter when cell membrane integrity is lost and thereby indicate apoptotic cells. - intracellular cytokine production: stain the cells with antibody-coupled fluorophore and permeabilize the cells so that antibodies can bind to intracellular cytokines. - FASC: cell sorting by giving cells in droplets different charges and attracking (or repelling) the droplets with deflection plates and thereby sorting them in different tubes.