How is neurotransmission in cholinergic neuron?

Neurotransmission occurs in six steps: Synthesis of Ach (choline transported from ECM to cytoplasm by energy-dependent carrier system that cotransports Na, cannot diffuse through membrane since + charge, can be inhibited by hemicholinium drug, uptake of choline is rate-limiting step of synthesis, then choline acetyl-transferase catalyzes rxn w/acetyle CoA to make acetylcholine) Storage (packaged in presynaptic vesicles by active transport, vesicle contains ATP and proteoglycan, which are potential cotransmitters, vesilces are called varicosities along nerve terminal) Release of Ach (Voltage gated Ca channels on presynaptic membrane open, causes increase in concentration of intracellular Ca promote fusion of vesicles with cell membrane are release of Ach, can be blocked by botulinum toxin, black widow spider venom can cause release of all Ach stored in synaptic vesicles to the space) Binding to receptors (Binds postsynaptic receptors or presynaptic ones that released it, two classes postsynaptic Musc and Nic) Degradation of Ach (Acetylecholinesterase cleaves Ach into choline and acetate in synaptic cleft, there is also pseudocholinesterase in plasma but no role Recycling of choline (recaptured by Na-coupled transporters