How is collection, processing and storage in HSCT?

Goal is to transfer hematopoietic stem cells (HSC) along with appropriate doses of key immune cells from donor to recipient Cells types that matter and are often measured CD34+ cells T-cells (CD3) B-cells (CD19) NK Cells (CD16, CD56) Cell numbers that are used to choose doses CD 34 cells/kg recipient weight Total Nucleated Cells (TNC) or mononuclear cells (MNC)/kg recipient weight Progenitor sources (bone marrow, peripheral stem cells, umbilical cord)