How is management of kidney disease in pathology?

➢ It is necessary to obtain at least two fragments one for morphological study under light microscopy and the other for immunofluorescence tests ➢ The conventional study using paraffin-fixed tissue: Reveals elementary kidney lesion, but needs the Use of special stains in addition to the standard HE staining : Masson's trichrome : Hyaline deposits, fibrosis, … Periodic Acid Schiff (PAS) : BM Silver Stain : Memranous deposits ➢Numerous cut sections are needed to highlight segmental and focal lesions ➢ Immunofluorescence studies : ✓Performed on frozen tissue ✓Reveals only extracellular deposits ✓Often gives precious information about the etiology of the disease ✓Antibodies used on every kidney biopsy : Heavy Chain immunoglobulines (A,G,M) Light Chain immunoglobulines (Kappa et lambda) complement (C3 et C1q) Fibrinogene