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N5 Biology - Unit 1: Cell Biology


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[Front]


What is a stain?
[Back]


A substance used to make cell structures more clearly visable.

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N5 Biology - Unit 1: Cell Biology - Details

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111 questions
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What is a stain?
A substance used to make cell structures more clearly visable.
Light microscope
A microscope that uses light
Compound microscope
A microscope that uses multiple lenses
Function of nucleus
Contains cell chromosomes which are made of DNA, these hold the genetic information which controls cell activity
Function of mitochondria
Site of aerobic respiration, mitochondria are found in greater abundance in cells with high energy demands such as muscle cells involved in respiration to release energy in both animal and plant cells
Function of cell membrane
Consists of phospholipid and protein molecules, selectively permeable, controls the entry and exit of materials in and out of the cell.
Function of ribosome
Site of protein synthesis, found in the cytoplasm or attatched to tubular structures in the cell
Function of chloroplast
Site of photosynthesis, contain green pigment chlorophyll which absorbs light energy
Function of cell wall
Gives support to the cell and structure to plant tissue, made of a carbohydrate called cellulose
Function of cytoplasm
Jelly like material, chemical reactions happen, contains organelles
Function of vacuole
Contains cell sap which is a solution of salts and sugars, important in maintaining the shape of the cell
Organelles
Small sub-cellular structures with a special function
Differences of a fungal cell
Only difference from an animal cell is the presence of a cell wall, this cell wall is not made from cellulose
Differences of a bacterial cell
No nucleus, has DNA and circular plasmids, the cell wall is different to plants
Selectively permeable meaning
It allows some molecules to pass from one side to the other while other molecule are unable to pass through it
Examples of molecules that move by diffusion
Carbon dioxide, oxygen, glucose
What is a bilayer?
A double layer of closely packed atoms or molecules
Fluid Mosaic
Describes how a cell membrane looks and functions
Test for glucose
Benedict's reagent turns brick red
Why do cells have a membrane?
So they can have different intracellular conditions from extracellular conditions
Why transport? (in green plant cells)
They need to photosynthesise so they require carbon dioxide to enter the cell
Passive transport
High to low concentration, does not require energy
Active transport
Low to high concentration, does require energy
Passive transport examples
Diffusion and osmosis
Diffusion
The movement of particles from an area of high concentration to an area of low concentration until evenly spread
Result of diffusion
Even distribution of that type of particle
Osmosis
The movement of water from an area of high water concentration to an area of low water concentration through a selectively permeable membrane
Fully turgid
Large vacuole, cell membrane pushed against cell wall, cell wall prevents cell from bursting
When is a cell plasmolysed?
In a salt solution (in a concentrated solution)
Plasmolysed
Small vacuole, cell membrane shrunken away from cell wall, cell wall prevents collapse
What happens to a red blood cell in water?
Water would enter the cell by osmosis, it would swell up then burst
Hypertonic
A solution which effectively has a LWC because there is a lot of solute dissolved in it
Hypotonic
A solution which effectively has a HWC because there is very little solute dissolved in it
Isotonic
Refers to two solutions having the same water concentration
What are the terms hypertonic, hypotonic and isotonic used for?
To compare water concentrations of fluids on either side of an SPM
Solute
The thing that gets dissolved
Solvent
What the solute gets dissolved in - the liquid that does the dissolving
Solution
The product of a solute dissolving in a solvent
Why does amoeba not burst?
It has a contractile vacuole which sucks up the water and squirts it back out
Why can't the DNA leave the nucleus?
It could get lost or damaged
Why can't the DNA leave the nucleus?
It could get lost or damaged
Why can't the DNA leave the nucleus?
It could get lost or damaged
Chromosomes description
Thread like structures found in the nucleus that contain genetic information, they are made up of long coiled strands of DNA
Genes description
Sections of DNA containing coded instructions for making a specific protein, each chromosome contains multiple genes which can be seen as a band pattern in chromosomes which have been stained
What are the rungs of the DNA ladder?
DNA bases - it's the DNA bases that hold the genetic code
What are DNA bases?
Chemical units which bond as complemetery base pairs to hold the double helix together
What is the backbone of DNA made of?
Sugar phosphate backbone
Base pair
Small chemical units which make up DNA joined like rungs on a ladder
DNA
Chemical which contains the genetic code
Gene
Unit of inheritance which controls a characteristic
Chromosome
Thread like structures found in the nucleus
Nucleus
Organelle in cells which contains genetic information
Cell
Basic unit of living things
Organism
Living being made of single or many cells
What are proteins?
Long chains of amino acids folded into a specific shape
What is mRNA?
A copy of the DNA sequence
What does mRNA do?
Takes the copied instructions out of the nucleus and into the cytoplasm, it is 'read' by the ribosome which uses the code to make the protein from amino acids
Why can't the DNA leave the nucleus?
It could get lost or damaged
What elements make up proteins?
Carbon, hydrogen, oxygen, nitrogen
What do proteins make the structure of?
Cells:cytoplasm, nucleus, cell membranes and enzymes
Proteins are an important part in ...
Muscle, hair, fingernails, horns, antlers
What are the 2 types of protein?
Structural and functional
Structural protein
Form part of a specific structure
Functional protein
Play an active part in something
Examples of structural proteins
Muscle, skin, bone, hair, antler, fur
Examples of functional proteins
Antibodies, enzymes, protein channels, receptors
What is a hormone?
Chemical messengers that are produced in one part of the body and have their effect on another part
Denatured
The shape of the active site of the enzyme changes so it is no longer complementary to its substrate
What happens if an enzyme is heated?
Because it is a protein, heat will affect its structure - it will become denatured
What does an enzyme do? (energy)
Lowers the activation energy required for a reaction
Optimum temperature
The temp at which there is highest enzyme activity
How do bacteria contain genetic information?
A small circular plasmid and a large circular chromosome
What is the chromosome and the plasmid made from?
Genes, each one coding for a different protein
What is genetic engineering?
Scientists can now remove genes from the plasmid and also insert specific genes from other organisms into it.
Stage 1 of genetic engineering
Identify the section of DNA that contains the required gene from the source chromosome
Stage 2 of genetic engineering
Extract the required gene using a restriction endonuclease enzyme
Stage 3 of genetic engineering
Insert the required gene into a bacterial plasmid using a ligase enzyme, the plasmid is the vector (carrier of genetic info)
Stage 4 of genetic engineering
Insert the plasmid into a host cell (a bacterium)