3 types of proteins that distinguished based on how they associate with membranes; understand how experimentally these 3 types can be identified, what forces connect these proteins with lipid bilayer?

Peripheral - released by changes in pH or ionic strength, removal of Ca2+ by a chelating agent, or addition of urea or carbonate Integral - extractable with detergents; proteins covalently attached to membrane lipids could be extracted with phospholipases Amphitropic proteins –association with membranes is regulated (found both in membranes and in cytosol).