Wet mount preparation of lymph node aspirate and chancre fluid are used as rapid methods for demonstration of trypanosomes. o Biopsies from liver, spleen, bone marrow, and lymph nodes are taken in visceral leishmaniasis for demonstration of Leishman Donovan (LD) bodies. o All biopsy tissues must be submitted to the laboratory without the addition of formalin fixative. If there is delay in transport or processing, the specimen should be placed in polyvinyl alcohol fixative o Adult filarial worms can sometimes be found in section of biopsied lymph node. o Corneal scrapings are useful in diagnosis of acanthamoeba keratitis.