FS HN 420: Example flow cytometry method: DNA and protein in yeast cells

Question

Example flow cytometry method: DNA and protein in yeast cells

Kate Jaros · Accepted Answer

1. grow yeast and fix cells
2. incubate in RNAase solution for 1 hour
3. Stain with dyes. Fluorescein isothiocyanate for protein, propidium iodide for DNA
4. Wash cells and suspend in buffer
5. Test in flow cytometer

FS HN 420

Example flow cytometry method: DNA and protein in yeast cells

1. grow yeast and fix cells 2. incubate in RNAase solution for 1 hour 3. Stain with dyes. Fluorescein isothiocyanate for protein, propidium iodide for DNA 4. Wash cells and suspend in buffer 5. Test in flow cytometer

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