Oceanography L11-15: Are there any problems associated with these molecular approaches?

Question

Are there any problems associated with these molecular approaches?

Alex Rapai · Accepted Answer

i) bias in cloning : extraction of DNA; genome size; no. of rRNA genes; unclonable fragments. 
ii) Coverage of clone libraries - Coverage C= 1 – (n1/N). Coverage is the portion of a clone library of infinite size that would be sampled by an actual clone library.

Oceanography L11-15

Are there any problems associated with these molecular approaches?

I) bias in cloning : extraction of DNA; genome size; no. of rRNA genes; unclonable fragments. ii) Coverage of clone libraries - Coverage C= 1 – (n1/N). Coverage is the portion of a clone library of infinite size that would be sampled by an actual clone library.

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