Question:
What are different protein labelling techniques?
Author: Mirte SchattorieAnswer:
Stable isotope labelling metabolic (SILAC): label introduced in first stage of analysis, very accurate labelling. chemical labelling: an isotope coded affinity tag (ICAT) is added to cysteines. isobaric peptide tagging: labelling is done after protein extraction and denaturation (all tags have identical mass).
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