What is Coomb's test

Direct Coombs Test: This test detects antibodies (Abs) on the surface of red blood cells (RBCs). It is utilized when suspecting hemolytic anemia due to various causes such as infection, autoimmune diseases, or reactions to medications. Coombs reagent is added to detect Abs bound to RBCs. A positive result indicates hemolysis. Indirect Coombs Test: This test identifies Abs in the patient’s serum. RBCs are mixed with patient serum, followed by the addition of secondary Abs. This is performed prior to transfusion to determine compatibility. It is crucial especially in cases where the patient may have Abs against the donor’s blood due to blood group difference